Coding

Part:BBa_K4144040:Design

Designed by: Pi Ruiqi   Group: iGEM22_WHU-China   (2022-10-04)


KanR with RBS to promote expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression of kanamycin resistance gene may be influenced especially in a large element control by one promoter out of design consideration. in order to guarantee the normal function of the gene, a RBS is added.


Source

The KanR coding sequence refers to pET-28a(+).

References